Antonio and Maria Parlavecchio, both 36, were indicted this week in federal court. Authorities say that at least three times, beginning in October 1998, Antonio Parlavecchio sent sperm from the Allenwood prison to a New York City fertility clinic, where his wife paid for storage.
Federal prosecutor Wayne P. Samuelson said he does not know whether attempts at artificial insemination were successful.
Four people, including three former prison guards, have already been indicted in the scheme.
Antonio Parlavecchio is serving time for loan sharking and racketeering, and federal criminal complaints list him as a mob associate.
He was cited this week for conspiracy, bribery and three counts of illegally possessing sperm kits for artificial insemination. His wife was charged with three counts of providing the kits.